Proteome Center Tuebingen (PCT) was founded in 2003 as a part of the Interdepartmental Institute for Cell Biology (IFIZ) and it has since established and applied methods for quantitative comparison of protein expression profiles, using state-of-the-art methodology and instrumentation.

Since December 2008, PCT is led by Prof. Boris Macek, who reports to Scientific Advisory Committee and Steering Committee. In addition, he has a teaching assignment at the Faculty of Biology, University of Tuebingen.   

PCT currently employs staff of 10 scientists and support personnel with core expertise in methods of protein analysis, such as different electrophoresis techniques and protein identification by mass spectrometry. PCT recently extended its expertise and shifted the emphasis to the workflows in the area of high accuracy peptide-based (“shot-gun”) quantitative proteomics that enable high dynamic range of protein detection. The quantitative workflows are mainly based on stable isotope labeling of cells and tissues and measurements of resulting protein digests using state-of-the-art LTQ-Orbitrap mass spectrometry. In addition, PCT possesses considerable infrastructure and expertise in protein/peptide separation technologies based on various analytical HPLC methods (strong cation exchange, reversed-phase chromatography, etc.), as well as bioinformatic analysis of shot-gun proteomics data.